mending material meaning in English

补炉料

According to these problems , we adopt to the method of mending material , optimize to fermentation media and partly ferment condition . finally , we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified . with the plasmid pbv220 - ifnr , pbv220 - hgfa , pbv220 - hgfb , pbv220 - hpk5 that expresses serve as the model , adopting the biostat - c15l of b . braun company , utilize the method of mending material to ferment , through optimization fermentation media and optimization partly ferment condition ( ventilate quantity , stir speed , mend material speed ) , eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室构建并稳定表达的重组质粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5为模型，分别从不同的表达宿主菌中筛选出一种适合大规模生产的菌种bl21 ( de3 ) ，该工程菌株连续传代100代表达质粒不丢失，表达量稳定；采用b . braun公司的biostat - c15l自控发酵罐，运用分批补料技术分别进行四种工程菌的高密度发酵，通过优化工程菌发酵的培养基配方及优化部分发酵条件(通气量、搅拌速度、补料速度) ，最终建立一种适于目的基因高效表达的高密度发酵工艺模式。